Comparative Genome Analysis and Assessment of the Functional Properties of Streptococcus thermophilus Strains

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Abstract

Streptococcus thermophilus are commonly used as starter cultures. Search for new safe strains with desired industrial and probiotic properties is an important issue. Complete genome sequencing was carried out, and the main genome characteristics of two new strains, S. thermophilus 16t (Str16t) and 159 (Str159), were determined. In silico analysis of the genomes revealed the absence of transmissible antibiotic resistance genes, virulence genes associated with pathogenicity, and integrated plasmids; gene clusters encoding class I and class II bacteriocin were found. In vitro tests showed phosphatase, peptidase, β-galactosidase, and esterase activity of both strains, as well as their ability to ferment glucose, lactose, sucrose, and ribose. Strain Str16t metabolized mannose as well. Str16t and Str159 are promising strains for application as starter and probiotic cultures.

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About the authors

K. V. Moiseenko

Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences

Email: fedorova_tv@mail.ru
Russian Federation, Moscow

O. A. Glazunova

Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences

Email: fedorova_tv@mail.ru
Russian Federation, Moscow

O. S. Savinova

Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences

Email: fedorova_tv@mail.ru
Russian Federation, Moscow

T. V. Fedorova

Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences

Author for correspondence.
Email: fedorova_tv@mail.ru
Russian Federation, Moscow

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2. Fig. 1. Anvi'o diagram representing the comparative genomic analysis of S. thermophilus strains 16t (Str16t) and S. thermophilus 159 (Str159). Gene clusters (two inner rings) were ordered (inner dendrogram) according to their presence (solid) or absence (grey) in the genomes of each strain. The presence of functional annotations in each gene cluster is indicated in green on the ring, signed according to annotation type

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3. Fig. 2. Resistance of strains of S. thermophilus to different groups of antibiotics: β-lactams (Amp - ampicillin, Amx - amoxicillin, Oxa - oxacillin, PenG - penicillin G); Fos - fosfomycin; Aminoglycosides (Gen - gentamicin, KanA - kanamycin A, Neo - neomycin); Tetracyclines (Dox - doxycycline, Tet - tetracycline); Macrolides (Azm - azithromycin); Lincosamides (Lcm - lincomycin); Amphenicols (Chl - chloramphenicol); Fluoroquinolones (Lev - levofloxacin, Pef - pefloxacin)

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4. Fig. 3. Biochemical characterisation of S. thermophilus 16t and 159: a - evaluation of carbohydrate fermentation ability (green colour - positive reaction, red - negative reaction); b - enzymatic profile

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