Antimicrobial activity of silver proteinate and its changes during storage

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Аннотация

Background: In the context of the growing problem of antibiotic resistance, the selection of drugs with non-specific antimicrobial action is a relevant approach for treating upper respiratory tract infections. Silver protein, known for its antimicrobial properties, is used as an active ingredient in several medical formulations.

Aim: The work aimed to assess the effect of storage duration and temperature on the antimicrobial activity of the pharmaceutical preparation with the International Nonproprietary Name silver protein.

Methods: To conduct the experimental study, reference strains of Staphylococcus aureus (ATCC 6538), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 9027), Bacillus cereus (ATCC 14579), and Candida albicans (ATCC 10231) were used as test cultures, along with silver protein solutions stored at 22 °C and 7 °C. Inoculations were performed from day 0 to day 75 at 7-day intervals. Antimicrobial efficacy was assessed by comparing the diameters (mm) of microbial growth inhibition zones throughout the experiment.

Results: On day 30, the diameters of the inhibition zones were larger in the 7 °C group: S. aureus by 25.0% (p = 0.033), E. coli by 12.2% (p = 0.041), P. aeruginosa by 18.3% (p = 0.042), B. cereus by 10.0% (p = 0.005), and C. albicans by 42.7% (p = 0.016). On days 37, 45, 53, 60, 68, and 75 of the study, the diameters of the inhibition zones for S. aureus, P. aeruginosa, B. cereus, and C. albicans remained unchanged (9, 8, 7, and 7 mm, respectively); for E. coli, on days 37, 45, 53, and 60, the diameters remained the same as on day 30 (8 mm), whereas on days 68 and 75 a decrease to 7 mm was observed (12.5%, p = 0.005).

Conclusion: Keeping the solution at 7 °C for 30 days does not reduce its antimicrobial activity, making it possible to choose a convenient storage option for the working solution. Given the preservation of antimicrobial activity through day 75, silver protein can be recommended for treating superinfections and chronic forms of rhinosinusitis.

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Авторлар туралы

Elena Kotelevets

Ryazan State Medical University

Хат алмасуға жауапты Автор.
Email: kotelevetse@mail.ru
ORCID iD: 0000-0001-7972-5861
SPIN-код: 1609-1183

MD, Cand. Sci. (Medicine)

Ресей, Ryazan

Irina Vorobyova

Ryazan State Medical University

Email: francais64@mail.ru
ORCID iD: 0000-0002-9113-9184
SPIN-код: 9216-5887

Cand. Sci. (Biology), Assistant Professor

Ресей, Ryazan

Valery Kiryushin

Ryazan State Medical University

Email: v.kirushin@rzgmu.ru
ORCID iD: 0000-0002-1258-9807
SPIN-код: 2895-7565

MD, Dr. Sci. (Medicine), Professor

Ресей, Ryazan

Әдебиет тізімі

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2. Fig. 1. Diameters of growth suppression zones of microbial strains with silver proteinate aqueous solution stored at 22 °С on the 45th (a) and 60th (b) days of the study.

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3. Fig. 2. Diameters of growth suppression zones of microbial strains with silver proteinate aqueous solution stored at 7 °С on the 53th day of the study.

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© Kotelevets E.P., Vorobyova I.V., Kiryushin V.A., 2024

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