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Calcium-activated chloride channels. Role of potassium ions

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1. 标题 文档的标题 Calcium-activated chloride channels. Role of potassium ions
2. 创建者 作者姓名，工作单位，国家 V. Zamoysky; Institute of Physiologically Active Compounds of the Russian Academy of Sciences; 俄罗斯联邦
2. 创建者 作者姓名，工作单位，国家 A. Gabrelian; Institute of Physiologically Active Compounds of the Russian Academy of Sciences; 俄罗斯联邦
2. 创建者 作者姓名，工作单位，国家 V. Grigoriev; Institute of Physiologically Active Compounds of the Russian Academy of Sciences; 俄罗斯联邦
3. 主题 学科
3. 主题 关键词 patch-clamp method; Purkinje cells of the cerebellum; calcium-activated chloride channels (CaCC); potassium ions
4. 描述 摘要

Using the patch-clamp method in the whole-cell configuration, it was shownthat external potassium ions play an important role in the regulation of calcium-activated chloride currents. A clear dependence of the amplitude of chloride currents on changes in the concentration of external potassium is shown. Changes in concentration of sodium, magnesium and calcium ions from membrane outside have no so significant effect, like outside potassium ions. The effect of potassium on the amplitudes of chloride currents is significantly greater than the effect it has on other cell ionic currents — sodium, potassium, cation. There is reason to believe that a change in the amplitudes of chloride currents contributes to the pathophysiological processes characteristic of hypokalemia and hyperkalemia.
5. 出版商 组织机构，地点 The Russian Academy of Sciences
6. 合作者 主管
7. 日期 (YYYY-MM-DD) 12.10.2024
8. 类型 现状与流派 同行评议的文章
8. 类型 类型 来源文章
9. 格式 文件格式
10. 识别码 环球资源指标 https://kld-journal.fedlab.ru/1026-3470/article/view/647810
10. 识别码 Digital Object Identifier (DOI) 10.31857/S1026347024030082
10. 识别码 eLIBRARY Document Number (EDN) VAJJDC
11. 源 期刊/会议标题 ; 卷., 期. (年) Izvestiâ Akademii nauk. Rossijskaâ akademiâ nauk. Seriâ biologičeskaâ; 编号 3 (2024)
12. 语言 English=en ru
13. 关系 补充文件 Fig. 1. a - series of depolarising pulses applied to the cell, from the fixation potential (-70 mV), in 10 mV steps, to +30 mV at the membrane, in whole-cell configuration; b - recorded response of cell currents to depolarising pulses; c - currents appearing at the end of depolarising pulses (108KB)
Fig. 2. Effect of different potassium concentrations in the outer solution on the amplitude of chlorine currents. The intra-pipette solution contains 120 mM potassium. Fixation potential (-70 mV). a - integral cell response in solution with [K+]nar. = 0 mM (n= 9); b - response in solution with [K+]nar. = 5 mM (n= 20); c - response in solution with [K+]nar. = 9 mM (n= 7); d - response in solution with [K+]nar. = 15 mM (n= 4); e - plots of volt-ampere characteristics plotted for CAHT at different values of [K+]nar. On the abscissa axis - current value in nanoamperes, on the ordinate axis - membrane potential in millivolts. Squares - [K+]nar. = 0 mM, circles - [K+]nat. = 5 mM, triangles - [K+]nat. = 9 mM, inverted triangles - [K+]nar. = 15 mM (112KB)
Fig. 3. Effect of different potassium concentrations in the external solution on the amplitude of inward and outward currents in Purkinje neurons. a - plot of the dependence of the amplitude of fast sodium currents on [K+]nar. = 0 mM (circles), [K+]nar. = 5 mM (squares) and [K+]nar. = 9 mM (triangles). VPR with potassium; b, plot of the dependence of the amplitude of fast sodium currents on [K+]nar. = 0 mM (circles), [K+]nar. = 5 mM (squares) and [K+]nar. = 9 mM (triangles). VPR with caesium; c, plot of the dependence of the amplitude of the maximum outgoing current on [K+]nar. = 0 mM (circles), [K+]nar. = 5 mM (squares) and [K+]nar. = 9 mM (triangles). VPR with potassium; d, plot of the dependence of the amplitude of the maximum outgoing current on [K+]nar. = 0 mM (circles), [K+]nar. = 5 mM (squares) and [K+]nar. = 9 mM (triangles). VPR with caesium (189KB)
Fig. 4. Effect of different potassium concentrations in the external solution on the amplitude of chlorine currents; VPR contains 120 mM caesium. a - whole-cell currents at [K+]nar. = 0 mM; b - whole-cell currents at [K+]nar. = 5 mM; c - whole-cell currents at [K+]nar. = 9 mM; d - whole cell currents at [K+]nar. = 15 mM; e - plot of volt-ampere characteristics of CAHT at [K+]nar. = 0 mM (circles); at [K+]nar. = 5 mM (squares); at [K+]nar. = 9 mM (triangles); at [K+]nar. = 15 mM (rhombuses) (127KB)
Fig. 5. Effect of external solution cations on the amplitude of CACT. a - effect of sodium ions (black squares - 140 mM sodium outside, black circles - 0 mM sodium outside) WRP with caesium; b - different concentration of magnesium ions (squares - 2 mM, circles - 5 mM, triangles - 9 mM) WRP with caesium; c - external calcium (squares - 2 mM, circles - 5 mM) WRP with potassium (162KB)
Fig. 6. CACT amplitudes. a - at different potassium ion concentrations on the outer side (circles - [K+]nar. = 0 mM, squares - [K+]nar. = 5 mM, triangles - [K+]nar. = 15 mM); b - at different concentrations of caesium ions from the outer side (circles - [Cs+]nar. = 5 mM, squares - [Cs+]nar. = 9 mM, triangles - [Cs+]nar. = 15 mM) (119KB)
Fig. 7. Effects of low (a) and high (b) concentrations of potassium ions on the outside of the membrane of a single cell. A1 - [K+]nar. = 0 mM, A2 - [K+]nar. = 1 mM, A3 - [K+]nar. = 2 mM, A4 - [K+]nar. = 3 mM, A5 - [K+]nar. = 5mM; B1 - [K+]nat. = 5mM, B2 - [K+]nat. = 9mM, B3 - [K+]nat. = 15 mM, B4 - [K+]nat. = 24 mM (224KB)
14. 范围 地理位置、年代时期、调查样本（性别、年纪等等）
15. 权力 版权及权限 版权所有 © Russian Academy of Sciences, 2024