Generation of LEPR K nockout Rabbits with CRISPR/CAS9 System

Мұқаба

Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Тек жазылушылар үшін

Аннотация

In humans mutations in the LEPR gene, encoding leptin hormone receptor, are associated with the development of morbid obesity, lipid metabolism dysregulation, defects of fertility. In rodents spontaneous mutations in Lepr gene have been described. Lepr knock-out (KO) animals have been generated using classical KO technology or CRISPR/Cas9 system. In rodents lipid metabolism is significantly different from that in humans or rabbits, therefore rabbits are considered as the most relevant model of morbid obesity and lipid metabolism dysregulation in humans, but so far LEPR knock-out rabbits have not been reported. Here using CRISPR/Cas9 system we generated LEPR knock-out rabbit by introducing a deletion of the region around exon 10 of the LEPR gene. We showed that the body weight of the knock-out rabbit was significantly higher than an average body weight of the wild type rabbits. CRISPR/Cas9-mediated technology for the generation of the LEPR knock-out rabbits will allow for the development of the model of morbid obesity and endocrine defects in humans due to leptin receptor mutations.

Негізгі сөздер

Авторлар туралы

Y. Silaeva

Institute of Gene Biology Russian academy of sciences

Хат алмасуға жауапты Автор.
Email: mshepelev@mail.ru

Center for Precision Genome Editing and Genetic Technologies for Biomedicine

Ресей, Moscow

P. Safonova

Institute of Gene Biology Russian academy of sciences

Email: mshepelev@mail.ru
Ресей, Moscow

D. Popov

Afanas`ev Research Institute of Fur – Bearing Animal Breeding and Rabbit Breeding

Email: mshepelev@mail.ru
Ресей, Rodniki, Moscow region

М. Filatov

Institute of Gene Biology Russian academy of sciences

Email: mshepelev@mail.ru

Center for Precision Genome Editing and Genetic Technologies for Biomedicine

Ресей, Moscow

Y. Okulova

Institute of Gene Biology Russian academy of sciences

Email: mshepelev@mail.ru

Center for Precision Genome Editing and Genetic Technologies for Biomedicine

Ресей, Moscow

R. Shafei

Moscow State University

Email: mshepelev@mail.ru
Ресей, Moscow

О. Skobel

Afanas`ev Research Institute of Fur – Bearing Animal Breeding and Rabbit Breeding

Email: mshepelev@mail.ru
Ресей, Rodniki, Moscow region

D. Vysotskii

Afanas`ev Research Institute of Fur – Bearing Animal Breeding and Rabbit Breeding

Email: mshepelev@mail.ru
Ресей, Rodniki, Moscow region

Y. Gubarev

Belgorod National Research University

Email: mshepelev@mail.ru
Ресей, Belgorod

V. Glazko

Afanas`ev Research Institute of Fur – Bearing Animal Breeding and Rabbit Breeding

Email: mshepelev@mail.ru
Ресей, Rodniki, Moscow region

T. Glazko

Afanas`ev Research Institute of Fur – Bearing Animal Breeding and Rabbit Breeding

Email: mshepelev@mail.ru
Ресей, Rodniki, Moscow region

P. Georgiev

Institute of Gene Biology Russian academy of sciences

Email: mshepelev@mail.ru

Academician of the RAS

Ресей, Moscow

G. Kosovsky

Afanas`ev Research Institute of Fur – Bearing Animal Breeding and Rabbit Breeding

Email: mshepelev@mail.ru
Ресей, Rodniki, Moscow region

М. Shepelev

Institute of Gene Biology Russian academy of sciences

Email: mshepelev@mail.ru

Center for Precision Genome Editing and Genetic Technologies for Biomedicine

Ресей, Moscow

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