Effect of combined use of melatonin and diethyldithiocarbamate on the N1E-115 cell line (a clone of cells from mouse neuroblastoma C-1300)

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Abstract

A study has been made on the effects of sodium diethyldithiocarbamate and melatonin and their interaction on proliferative activity, the variation in cytosolic Ca2+, membrane potential and reactive oxygen species production in the neuroblastoma N1E-115 cell line (a clone of cells from mouse neuroblastoma C-1300). This study showed that combined use of diethyldithiocarbamates and melatonin inhibited proliferation and enhanced cell differentiation. At the same time, the content of Bcl-2 decreased, while the content of Bax increased, that were likely to trigger an apoptotic cascade. However, the use of these two drugs in combination did not enhance the observed effects. Therefore, this study suggests that the mechanisms by which diethyldithiocarbamates and melatonin act are apparently different.

About the authors

S. N Myakisheva

Institute of Cell Biophysics, Russian Academy of Sciences

Email: myakisheva@mail.ru
Pushchino, Moscow Region, Russia

Y. L Baburina

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences

Pushchino, Moscow Region, Russia

M. I Kobyakova

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences

Pushchino, Moscow Region, Russia

R. R Krestinin

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences

Pushchino, Moscow Region, Russia

L. D Sotnikova

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences

Pushchino, Moscow Region, Russia

O. V Krestinina

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences

Email: ovkres@mail.ru
Pushchino, Moscow Region, Russia

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