Hepatopancreatic Proteins of Snow Crab and Red King Crab with Antibacterial Activity

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Abstract

Troughout their long history, crustaceans, despite lacking a highly specific vertebrate-like adaptive immune system, have successfully adapted to living within their environment rich in microorganisms, in part due to the presence of antimicrobial peptides. One valuable source of antimicrobial peptides is hepatopancreas, a waste product from crab fishery and its processing. Data from zymogram and 1H NMR spectra showed that the extract of snow crab hepatopancreas contains a small peptide (about 3 kDa) that hydrolyzes the cell wall and the cell wall polysaccharide of M. lysodeikticus. The discovered peptide may be of interest for practical application. A protein (about 14 kDa), isolated from the red king crab hepatopancreas using affinity chromatography on heparin-Sepharose, is also active against the cell wall of the gram-positive bacterium M. lysodeikticus, as it was demonstrated with zymography and turbidimetric methods.

About the authors

V. G Molchanov

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences

Pushchino, Russia

A. Y Yegorov

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences

Pushchino, Russia

D. A Osetrina

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences

Pushchino, Russia

V. Yu Novikov

Polar Branch of Russian Federal Research Institute of Fisheries and Oceanography

Murmansk, Russia

N. M Novojilov

M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Moscow, Russia

A. A Timchenko

Institute of Protein Research, Russian Academy of Sciences

Pushchino, Russia

E. A Sogorin

Institute for Biological Instrumentation, Russian Academy of Sciences

Pushchino, Russia

M. A Timchenko

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences

Email: maria_timchenko@mail.ru
Pushchino, Russia

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