Comparative Evaluation of Interaction Force Characteristics for the Lipopolysaccharide of Yersinia pseudotuberculosis and Antibodies by Optical Trapping and Atomic Force Microscopy

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Abstract

Optical tweezers and atomic force microscopy were used for comparative evaluation of the interaction force between the lipopolysaccharide of Yersinia pseudotuberculosis and monoclonal antibodies. This paper discusses the peculiarities of two methods which allow determining significant differences in the values of the measured force required to rupture the interaction of probe sensitized by lipopolysaccharide (polystyrene microsphere for optical tweezers and silicon nitride cantilever for atomic force microscopy) with substrate (glass and mica, respectively) covered with monoclonal antibodies. In atomic force microscopy, the cantilever slides along the substrate for some time after the piezo stage is brought to a stop, causing changes in the spatial structure of sensitins and, therefore, redistribution of multiple bonds between the lipopolysaccharide agglomerate and antibodies. No significant displacement of the microsphere along the substrate occurs when an optical tweezers setup is used, and, unlike atomic force microscopy, the breaking of a complex bond between lipopolysaccharide and complementary antibodies is recorded in the form of a single and short-term (1–2 ms) leap of the photodetector signal employing optical tweezers. The recorded values of the force required to rupture the interaction measured by both methods are relative and vary depending on the chosen experimental conditions. It is shown that the non-specific component of the force needed to break the interaction measured by atomic force microscopy is significantly higher than that determined with optical tweezers.

About the authors

A. A Byvalov

Vyatka State University; nstitute of Physiology, Кomi Science Centre of the Ural Branch of the Russian Academy of Sciences

Email: byvalov@nextmail.ru
Kirov, 610000 Russia; Syktyvkar, 167982 Russia

V. S Belozerov

Vyatka State University; nstitute of Physiology, Кomi Science Centre of the Ural Branch of the Russian Academy of Sciences

Kirov, 610000 Russia; Syktyvkar, 167982 Russia

I. V Konyshev

Vyatka State University; nstitute of Physiology, Кomi Science Centre of the Ural Branch of the Russian Academy of Sciences

Kirov, 610000 Russia; Syktyvkar, 167982 Russia

B. A Ananchenko

Vyatka State University

Kirov, 610000 Russia

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