Study of Cytotoxicity of Selenium Nanoparticles Synthesized Using Artificial Metal-Binding Tumor-Specific Protein W8-3C

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Abstract

For the first time, the pW8-3C construct encoding the artificial tumor-specific protein W8-3C with the addition of 3 residues of free Cys at the C end was created and described. Using purified W8-3C protein, dispersions of nanoparticles 75.24 nm in diameter at polydispersity index (Pdi) of 0.064 and Se content of 1566 µg/mL were obtained and characterized for the first time. The dispersions remained stable upon storage for 6 months at +4°C. For comparison, the maximum Se content in the nanoparticle dispersion obtained in the presence of W8-3C protein and Pluronic F-127 was 399 µg/ml. The cytotoxic activity of the obtained nanoparticles was studied on transplanted cells of human tumor lines: HeLa (cervical carcinoma), U-87 MG (glioblastoma), MCF-7 (breast carcinoma) and HCT-116 (colon carcinoma) and compared with that on the diploid human fibroblast line WI-38 in vitro. It was shown that the IC50 of Se nanoparticles obtained using the W8-3C protein for tumor lines ranged from 5.25 to 8.37 µg/ml, while the IC50 for normal fibroblasts was 14.3 µg/ml (difference in values by a factor of 1.7–2.7 times).

About the authors

N. V Pozdnyakova

N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation; N.I. Vavilov Institute of General Genetics, Russian Academy of Sciences

Moscow, 115522 Russia; Moscow, 119991 Russia

Yu. K Biryukova

N.M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences

Moscow, 119334 Russia

Z. A Sokolova

N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation

Moscow, 115522 Russia

M. A Baryshnikova

N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation

Moscow, 115522 Russia

E. S Shcherbakova

N.I. Vavilov Institute of General Genetics, Russian Academy of Sciences; Research Laboratory "Genetics", Kursk State University

Email: ledera@yandex.ru
Moscow, 119991 Russia; Kursk, 305000 Russia

M. S Smirnova

N.I. Vavilov Institute of General Genetics, Russian Academy of Sciences

Moscow, 119991 Russia

A. B Shevelev

N.I. Vavilov Institute of General Genetics, Russian Academy of Sciences

Moscow, 119991 Russia

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