Transcriptional Activity of CCA 1 in Northern Population Arabidopsis thaliana Plants under Altered Light Conditions

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Abstract

The dynamics of the transcriptional activity of one of the key genes of the circadian network, CCA1, was analyzed under conditions of a natural light photoperiod of a long day (16L : 8D) and under an inverted light regime (8D : 16L) in A. thaliana plants of the northern natural population (Karelia). It has been shown that under conditions of an inverted shift in the light regime, there is a sharp increase in the expression of this gene with a phase shift in the circadian rhythm by 2 hours. The level of CCA1 transcriptional activity was almost two times higher compared to the natural light conditions. At the same time, the endogenous rhythm of the gene was preserved, but with a smaller amplitude. With age, 30-day-old plants grown under inverted conditions experienced a loss of endogenous CCA1 circadian rhythm. The results obtained allow us to conclude that the circadian rhythms of A. thaliana, northern natural populations, probably play an important role in adaptation to changing light conditions, and that one of the key clock genes, CCA1, plays a significant role in this process.

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About the authors

M. V. Zaretskaya

Institute of Biology of Karelian Research Centre Russian Academy of Sciences

Author for correspondence.
Email: genmg@mail.ru
Russian Federation, Petrozavodsk, 185910

О. M. Fedorenko

Institute of Biology of Karelian Research Centre Russian Academy of Sciences

Email: fedorenko_om@mail.ru
Russian Federation, Petrozavodsk, 185910

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2. Fig. 1. Graphic representation of the conditions for growing plants under long daylight hours: (a) – seeds were sown under natural photoperiod (16L : 8D); light was on from 6 a.m. to 10 p.m.; 20-day-old plants (Ш20) were analyzed. (b) – plants from group “a” were grown for 10 days under inverted light conditions (8D : 16L); light was on from 5 p.m. to 9 a.m.; 30-day-old plants (Ш30) were analyzed. (c, d) – seeds were sown under inverted light conditions (8D : 16L); 20-day-old (C20) and 30-day-old (C30) plants were analyzed.

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3. Fig. 2. Dynamics of CCA1 transcriptional activity in 20-day-old A. thaliana plants of the Shuiskaya (Ш20) and Ler populations grown under natural photoperiod conditions of 16L:8D (light was turned on at 6 am). Note. Here and in Figs. 3 and 4: X-axis – time of day in hours; Y-axis – relative level of CCA1 transcripts.

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4. Fig. 3. Dynamics of CCA1 transcriptional activity in A. thaliana plants of the Shuiskaya population grown under inverted light conditions of 8D:16L (light was turned on at 5 p.m.). a – 30-day-old plants grew under inverted light conditions (Ш30) only for the last 10 days; b and c – 20-day-old (С20) and 30-day-old (С30) plants constantly grown under inverted light conditions.

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5. Fig. 4. Dynamics of CCA1 transcriptional activity in A. thaliana plants of the Shuyskaya population grown under inverted light conditions of 8D : 16L (light continues from 5 p.m. to 9 a.m.). a – plants grew under natural light photoperiod conditions (16L : 8D) (Ш20) (data are given from Fig. 1 for comparison); b – 30-day-old plants grew under inverted light conditions only for the last 10 days (Ш30); c and d – 20-day-old (С20) and 30-day-old (С30) plants constantly grown under inverted light conditions.

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