Molecular and morphological description of Diplostomum spathaceum metacercariae from Abramis brama l. Of Lake Syamozero (North-West Russia)

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Trematodes Diplostomum von Nordmann, 1832 are widely distributed parasites with complex life cycles involving freshwater snails as the first intermediate hosts, various fish species as the second intermediate hosts, and fish-eating birds as definitive hosts. Metacercariae of Diplostomum spp. are important fish pathogens with problematic morphological identification. The present research of Diplostomum larvae in the eyes of the freshwater bream Abramis brama was carried out within the framework of the long-term parasitological monitoring of fish in Lake Syamozero. The study has provided molecular and morphological characterization of Diplostomum spathaceum metacercariae from the lens of the freshwater bream. The partial cox1 mtDNA sequences used for molecular identification of the isolates were identical with those obtained previously, but morphological features of the metacercariae matched the literature data only in part. Comparison of different dimensions of parasites by discriminant analysis suggests that Diplostomum rutili is a junior synonym of Diplostomum spathaceum. New and archival data on diplostomids from bream’s eyes demonstrate a long-term increase in the infection prevalence.

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作者简介

D. Lebedeva

Institute of Biology, Karelian Research Centre of the Russian Academy of Sciences

编辑信件的主要联系方式.
Email: daryal78@gmail.com
俄罗斯联邦, 11 Pushkinskaya St., Petrozavodsk, 185910

I. Sukhovskaya

Institute of Biology, Karelian Research Centre of the Russian Academy of Sciences

Email: daryal78@gmail.com
俄罗斯联邦, 11 Pushkinskaya St., Petrozavodsk, 185910

A. Kochneva

Institute of Biology, Karelian Research Centre of the Russian Academy of Sciences

Email: daryal78@gmail.com
俄罗斯联邦, 11 Pushkinskaya St., Petrozavodsk, 185910

L. Lysenko

Institute of Biology, Karelian Research Centre of the Russian Academy of Sciences

Email: daryal78@gmail.com
俄罗斯联邦, 11 Pushkinskaya St., Petrozavodsk, 185910

N. Kantserova

Institute of Biology, Karelian Research Centre of the Russian Academy of Sciences

Email: daryal78@gmail.com
俄罗斯联邦, 11 Pushkinskaya St., Petrozavodsk, 185910

D. Zaitsev

Petrozavodsk State University

Email: daryal78@gmail.com
俄罗斯联邦, 33 Lenin Ave., Petrozavodsk, 185910

N. Milyanchuk

Institute of Biology, Karelian Research Centre of the Russian Academy of Sciences

Email: daryal78@gmail.com
俄罗斯联邦, 11 Pushkinskaya St., Petrozavodsk, 185910

E. Ieshko

Institute of Biology, Karelian Research Centre of the Russian Academy of Sciences

Email: daryal78@gmail.com
俄罗斯联邦, 11 Pushkinskaya St., Petrozavodsk, 185910

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2. Figure 1. Sampling location: Lake Syamozero. Syargilahta Bay is marked with a black circle.

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3. Figure 2. Bayesian inference (BI) and maximum likelihood (ML) analyses tree for Diplostomum spp. based on the partial cox1 mtDNA sequences (384 bp). Nodal supports from both analyses are indicated as BI/ML. The scale bar indicates the expected number of substitutions per site. Only significant values of the posterior probabilities (≥ 0.5) are indicated. Newly obtained sequences are in bold. Abbreviations: ad – adult, mtc – metacercariae, Ab – Abramis brama, Bb – Blicca bjoerkna, Cm – Cyprinion macrostomum, La – Larus argentatus, Pp – Pungitius pungitius, Rr – Rutilus rutilus, Sa – Salvelinus alpinus, Ch – China, E – Estonia, G – Germany, H – Hungary, I – Iraq, Ic – Iceland, R – Russia, S – Slovakia, Sp – Spain.

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4. Figure 3. Haplotype network for Diplostomum spathaceum based on novel and published partial cox1 mtDNA sequences of Kudlai et al. (2017). Unsampled intermediate haplotype is represented by a short intersecting line; each branch corresponds to a single mutational difference and connective lines represent one mutational step. Circle size is proportional to the number of isolates sharing a haplotype; black circles indicate transitive haplotypes that have not been found.

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5. Figure 4. Metacercariae of Diplostomum spathaceum with different shape of body from the lens of Abramis brama in Lake Syamozero. Scales: 100 μm.

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6. Figure 5. Ordination of the traits of Diplostomum spathaceum and D. rutili metacercariae by discriminant analysis. The mean values of nine traits, including morphometry, from the present study and the literature were involved: 1 – green rhombus: D. spathaceum (Shigin, 1986); 2 – black asterisk: D. spathaceum (Niewiadomska, 2010); 3 – blue cross: D. spathaceum (Faltýnková et al., 2014); 4 – yellow oval: D. spathaceum (Pérez-del-Olmo et al., 2014); 5 – navy square: D. spathaceum (Kudlai et al., 2017); 6 – pink triangle: D. rutile (Shigin, 1986); 7 – black circle: means of dimensions of D. spathaceum from Lake Syamozero (the coloured points – red, light blue, dark blue, green, brown, purple – represent the specimens taken from different host individuals).

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7. Figure 6. Long-term changes of Diplostomum sp. metacercariae infection parameters in eyes of the bream Abramis brama in Lake Syamozero.

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