Dominant Bacterial Taxa of Chernozems and Factors Affecting Their Abundance in the Bacterial Community

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Abstract

Families and genera of bacteria that dominate in the chernozems of the forest-steppe zone have been identified. Microbiological profiling of samples of arable and non-arable chernozems using the 16S rRNA gene sequencing was carried out in different phases of the vegetation period: in June and August. The changes in the proportion of individual bacterial families depending on land use and time are shown. Correlations between the prevalence of bacterial families and the chemical parameters of the soil have been revealed. The predominant role of nitrates in the formation of the community structure, in this process the content of organic carbon, soil moisture and its pH play an important role. Despite the revealed differences in the proportions of the studied families depending on land use and the time of sample collection, the set of dominant bacterial families in the studied samples remained stable. The first 6 dominant families make up about 1/4 of the entire community, and the first 20 make up about 40%. The obtained results create prerequisites for further study of the variability of the taxonomic composition of the bacterial community of chernozems in various biotic and agrochemical conditions.

About the authors

K. S. Boyarshin

Belgorod State University

Author for correspondence.
Email: kboyarshin@mail.ru
Russian Federation, Belgorod

V. V. Adamova

Belgorod State University

Email: kboyarshin@mail.ru
Russian Federation, Belgorod

W. Zheng

Belgorod State University

Email: kboyarshin@mail.ru
Russian Federation, Belgorod

E. V. Nikitinskaya

Cherepovets State University

Email: kboyarshin@mail.ru
Russian Federation, Cherepovets

O. Yu. Obukhova

Belgorod State University

Email: kboyarshin@mail.ru
Russian Federation, Belgorod

M. V. Kolkova

Belgorod State University

Email: kboyarshin@mail.ru
Russian Federation, Belgorod

V. A. Nesterenko

Dokuchaev Soil Science Institute

Email: kboyarshin@mail.ru
Russian Federation, Moscow

O. S. Bespalova

Belgorod State University

Email: kboyarshin@mail.ru
Russian Federation, Belgorod

V. V. Klyueva

Belgorod State University

Email: kboyarshin@mail.ru
Russian Federation, Belgorod

K. A. Degtyareva

Belgorod State University

Email: kboyarshin@mail.ru
Russian Federation, Belgorod

L. V. Nesteruk

Belgorod State University

Email: kboyarshin@mail.ru
Russian Federation, Belgorod

Yu. N. Kurkina

Belgorod State University

Email: kboyarshin@mail.ru
Russian Federation, Belgorod

O. A. Makanina

Belgorod State University

Email: kboyarshin@mail.ru
Russian Federation, Belgorod

E. S. Ivanova

Belgorod State University

Email: kboyarshin@mail.ru
Russian Federation, Belgorod

Zh. Li

Dezhou University

Email: kboyarshin@mail.ru
China, Dezhou

I. V. Batlutskaya

Belgorod State University

Email: kboyarshin@mail.ru
Russian Federation, Belgorod

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2. Fig. 1. Sampling points on the soil map of Belgorod region of Russia

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3. Fig. 2. Chemical characteristics of arable (A) and non-arable (N/A) chernozems with their standard deviations: pH KCl, mass fraction of organic matter (Sorg, %), mass concentrations of exchangeable potassium in terms of K2O, exchangeable phosphorus in terms of P2O5, NO3- and NH4+ in terms of nitrogen

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