Characteristic parameters of unsaturated fatty acid residues upon liquid chromatography of lipids in media with silver ions

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Abstract

The results of liquid chromatography of a complex mixture of unsaturated lipid molecules as the basis of the hydrophobic matrix of biomembranes are summarized. The data of relative retention of such lipids, which included residues of the most important fatty acids, allowed calculating the most characteristic general parameters that satisfactorily determine their behavior when silver salt is introduced into a planar or column liquid chromatographic system in order to drastically increase the selectivity of separation of unsaturated lipid molecules from each other. A variant of quantitative estimation of the relationship between the level of selectivity of separation of particular molecules of natural lipids from each other and the proposed parameters of their constituent fatty acid residues, which are calculated on the basis of variations in the chemical potential of such molecules when silver appears in this system, is proposed.

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About the authors

V. P. Pchelkin

K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences

Author for correspondence.
Email: bas1953@mail.ru
Russian Federation, Moscow

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Supplementary files

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2. Fig. 1. Sections of the hydrophobicity scale of lipid molecules HBᵢ

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3. Fig. 2. Nominal levels of the main (ψUns, black columns) parameter of oleic (Ole), linoleic (Lin) and α-linolenic (αLnn) acid residues, as well as the values ​​of the complementary (εUns, white columns) and summing (λUns, grey columns) parameters [17, 18] according to Ag⁺-RP TLC of rac-1,2-Ole₂Gro, rac-1,2-Lin₂Gro and rac-1,2-Lnn₂Gro [14], as well as Ag⁺-RP HPLC of phenethyl and phenacyl esters of these acids [11].

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4. Fig. 3. Nominal levels of the ψUns parameter (gray columns) and the λUns values ​​of arachidonic (AchΔ₄), eicosapentaenoic (EPA) and docosahexaenoic (DHA) acid residues found in phenethyl and phenacyl esters (white and black columns, respectively [17, 18]) based on Ag⁺-RP HPLC data [11].

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