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Derivation of Induced Pluripotent Stem Cells Line (RCPCMi009-A-1) with Knockout of the UBE2A Gene by CRISPR/Cas9 Genome Editing
- Authors: Khomyakova E.A.1, Fedorenko A.V.1,2, Surdina A.V.1, Volovikov E.A.1,3, Belikova L.D.1,3, Zerkalenkova E.A.4, Lagarkova M.A.1,5, Bogomazova A.N.1,3,5
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Affiliations:
- Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine
- Skolkovo Institute of Science and Technology
- Research Institute of Medical Genetics, Tomsk National Research Medical Center of the Russian Academy of Scieces
- Rogachev Federal Scientific and Clinical Centre of Pediatric Hematology, Oncology and Immunology
- Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Lopukhin Federal Research and Clinical Center of Physical and Chemical Medicine
- Issue: Vol 54, No 6 (2023)
- Pages: 405-414
- Section: Collection of pluripotent stem cell lines
- URL: https://kld-journal.fedlab.ru/0475-1450/article/view/669914
- DOI: https://doi.org/10.31857/S0475145023060046
- EDN: https://elibrary.ru/HKXTMY
- ID: 669914
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Abstract
The deletions and mutations in the UBE2A gene cause X-linked mental retardation syndrome of Nascimento type first described in 2006 (Nascimento et al., 2006). To study the role of the UBE2A gene in neurodevelopment, we generated a human iPSC line with knockout of the UBE2A gene (RCPCMi009-A-1) using genome editing CRISPR/Cas9 technology. The knockout of the UBE2A gene was confirmed by Western blotting. The pluripotent state of the RCPCMi009-A-1 iPSCs line was confirmed by typical stem cell morphology, normal male karyotype maintenance, expression of pluripotency markers and the ability to differentiate into three germ layers.
About the authors
E. A. Khomyakova
Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine
Author for correspondence.
Email: kate.hom@gmail.com
Russia, 119435, Moscow
A. V. Fedorenko
Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine; Skolkovo Institute of Science and Technology
Email: kate.hom@gmail.com
Russia, 119435, Moscow; Russia, 121205, Moscow
A. V. Surdina
Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine
Email: kate.hom@gmail.com
Russia, 119435, Moscow
E. A. Volovikov
Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine; Research Institute of Medical Genetics, Tomsk National Research Medical Center of the Russian Academy of Scieces
Email: kate.hom@gmail.com
Russia, 119435, Moscow; Russia, 634050, Tomsk
L. D. Belikova
Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine; Research Institute of Medical Genetics, Tomsk National Research Medical Center of the Russian Academy of Scieces
Email: kate.hom@gmail.com
Russia, 119435, Moscow; Russia, 634050, Tomsk
E. A. Zerkalenkova
Rogachev Federal Scientific and Clinical Centre of Pediatric Hematology, Oncology and Immunology
Email: kate.hom@gmail.com
Russia, 117997, Moscow
M. A. Lagarkova
Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine; Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Lopukhin Federal Research and Clinical Center of Physical and Chemical Medicine
Email: kate.hom@gmail.com
Russia, 119435, Moscow; Russia, 119435, Moscow
A. N. Bogomazova
Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine; Research Institute of Medical Genetics, Tomsk National Research Medical Center of the Russian Academy of Scieces; Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Lopukhin Federal Research and Clinical Center of Physical and Chemical Medicine
Email: kate.hom@gmail.com
Russia, 119435, Moscow; Russia, 634050, Tomsk; Russia, 119435, Moscow
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