Мақаланы E-mail арқылы жіберу Generation of an Induced Pluripotent Stem Cell Line, ICGi042-A, by Reprogramming Peripheral Blood Mononuclear Cells from Parkinson’s Disease Patient with c.1000G>A Mutation in LRRK2 Gene
- Авторлар: Grigor’eva E.V.1,2,3, Kovalenko L.V.4, Rzaev J.A.5, Khabarova E.A.1,5, Vyatkin Y.V.6, Minina J.M.1, Medvedev S.P.1,2,3, Malakhova A.A.1,2,3, Pavlova S.V.1,2,3, Zakian S.M.1,2,3
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Мекемелер:
- Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences
- Meshalkin National Medical Research Center, Ministry of Health of the Russian Federation
- Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences
- Khanty-Mansiysk Autonomous Okrug – Ugra “Surgut State University”
- FSBI Federal Neurosurgical Center
- Novosibirsk State University
- Шығарылым: Том 54, № 1 (2023)
- Беттер: 87-95
- Бөлім: Collection of pluripotent stem cell lines
- URL: https://kld-journal.fedlab.ru/0475-1450/article/view/669970
- DOI: https://doi.org/10.31857/S0475145023010068
- EDN: https://elibrary.ru/FRFNVG
- ID: 669970
Дәйексөз келтіру
Ашық рұқсат
Рұқсат берілді
Тек жазылушылар үшін
Аннотация
The search for new polymorphisms associated with hereditary diseases is important for diagnostics and the study of the disease development pathology. We have analyzed clinical exome of a Parkinson’s disease patient and identified single-nucleotide variations in the LRRK2 (c.1000G>A, c.2167A>G) and PINK1 (c.1562A>C) genes. The LRRK2:c.1000G>A mutation has uncertain clinical significance, and is interesting for further investigation. We generated induced pluripotent stem cells (IPSCs) from PBMCs of the patient by a non-integrating episomal vectors. IPSCs demonstrate typical morphology and normal karyotype (46,XY), express pluripotency markers (OCT4, SOX2, NANOG, SSEA4, TRA-1-60), and are able to produce derivatives of three germ layers.
Негізгі сөздер
Авторлар туралы
E. Grigor’eva
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences; Meshalkin National Medical Research Center, Ministry of Health of the Russian Federation; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences
Хат алмасуға жауапты Автор.
Email: evlena@bionet.nsc.ru
Russia, 630090, Novosibirsk, prosp. acad. Lavrentieva, 10; Russia, 630055, Novosibirsk, ul. Rechkunovskaya, 15; Russia, 630090, Novosibirsk, prosp. acad. Lavrentieva, 8
L. Kovalenko
Khanty-Mansiysk Autonomous Okrug – Ugra “Surgut State University”
Email: evlena@bionet.nsc.ru
Russia, 628403, Surgut, prosp. Lenina, 1
J. Rzaev
FSBI Federal Neurosurgical Center
Email: evlena@bionet.nsc.ru
Russia, 630087, Novosibirsk, ul. Nemirovicha-Danchenko, 132/1
E. Khabarova
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences; FSBI Federal Neurosurgical Center
Email: evlena@bionet.nsc.ru
Russia, 630090, Novosibirsk, prosp. acad. Lavrentieva, 10; Russia, 630087, Novosibirsk, ul. Nemirovicha-Danchenko, 132/1
Y. Vyatkin
Novosibirsk State University
Email: evlena@bionet.nsc.ru
Russia, 630090, Novosibirsk, ul. Pirogova, 2
J. Minina
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences
Email: evlena@bionet.nsc.ru
Russia, 630090, Novosibirsk, prosp. acad. Lavrentieva, 10
S. Medvedev
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences; Meshalkin National Medical Research Center, Ministry of Health of the Russian Federation; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences
Email: evlena@bionet.nsc.ru
Russia, 630090, Novosibirsk, prosp. acad. Lavrentieva, 10; Russia, 630055, Novosibirsk, ul. Rechkunovskaya, 15; Russia, 630090, Novosibirsk, prosp. acad. Lavrentieva, 8
A. Malakhova
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences; Meshalkin National Medical Research Center, Ministry of Health of the Russian Federation; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences
Email: evlena@bionet.nsc.ru
Russia, 630090, Novosibirsk, prosp. acad. Lavrentieva, 10; Russia, 630055, Novosibirsk, ul. Rechkunovskaya, 15; Russia, 630090, Novosibirsk, prosp. acad. Lavrentieva, 8
S. Pavlova
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences; Meshalkin National Medical Research Center, Ministry of Health of the Russian Federation; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences
Email: evlena@bionet.nsc.ru
Russia, 630090, Novosibirsk, prosp. acad. Lavrentieva, 10; Russia, 630055, Novosibirsk, ul. Rechkunovskaya, 15; Russia, 630090, Novosibirsk, prosp. acad. Lavrentieva, 8
S. Zakian
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences; Meshalkin National Medical Research Center, Ministry of Health of the Russian Federation; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences
Email: evlena@bionet.nsc.ru
Russia, 630090, Novosibirsk, prosp. acad. Lavrentieva, 10; Russia, 630055, Novosibirsk, ul. Rechkunovskaya, 15; Russia, 630090, Novosibirsk, prosp. acad. Lavrentieva, 8
Әдебиет тізімі
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