Spleen Morphogenesis during the Neonatal Period in Rats Exposed to Endocrine Disruptor DDT

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Spleen morphogenesis during the neonatal period in rats exposed in prenatal and postnatal development to low doses of dichlorodiphenyltrichloroethane (DDT), a persistent universal pollutant with endocrine disrupting properties, was studied. More intensive formation of periarterial lymphoid sheaths and marginal zone and simultaneously decreased rate of B-cell differentiation in the spleen were revealed. A higher content of differentiating T-cells and a lower number of cytotoxic T-lymphocytes by the end of the first week of life indicates a decrease in the differentiation of the latter. A lower content of neutrophils in the marginal zone also indicates a delay in the rate of functional development of lymphoid tissue, as opposed to morphological, in rats developing under exposure to low doses of DDT.

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N. Yaglova

Avtsyn Research Institute of Human Morphology of the Petrovsky National Research Centre of Surgery

编辑信件的主要联系方式.
Email: yaglova@mail.ru
俄罗斯联邦, Moscow

B. Gagulaeva

Avtsyn Research Institute of Human Morphology of the Petrovsky National Research Centre of Surgery

Email: yaglova@mail.ru
俄罗斯联邦, Moscow

S. Obernikhin

Avtsyn Research Institute of Human Morphology of the Petrovsky National Research Centre of Surgery

Email: yaglova@mail.ru
俄罗斯联邦, Moscow

E. Timokhina

Avtsyn Research Institute of Human Morphology of the Petrovsky National Research Centre of Surgery

Email: yaglova@mail.ru
俄罗斯联邦, Moscow

V. Yaglov

Avtsyn Research Institute of Human Morphology of the Petrovsky National Research Centre of Surgery

Email: yaglova@mail.ru
俄罗斯联邦, Moscow

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2. Fig. 1. Changes in absolute (a) and relative (b) masses of the spleen in the first week of life in rats of the control group (1) and rats developing under the influence of low doses of DDT (2) (M ± m). ^ - statistically significant differences from the corresponding values on the 1st day of postnatal development, * - from the values of the control group (p < 0.01)

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3. Fig. 2. Characteristics of the white pulp in newborn rats of the control group and those developing under the influence of low doses of DDT: a - structure of the spleen of a newborn rat of the control group; b - structure of the spleen of a rat exposed to pre- and postnatal low doses of DDT. Arrows indicate the forming PALMs. Haematoxylin and eosin staining. Eq. 100; c - number of lymphoid couplings in the spleen slice (M ± m); d - thickness of lymphoid couplings (M ± m). * - from the values of the control group (p < 0.01)

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4. Fig. 3. Characteristics of the white pulp in 7-day-old rats of the control group and those developing under the influence of low doses of DDT (M ± m): a - share of white pulp in the spleen; b - share of PALM with marginal zone; c - number of cells in 1 mm2 of PALM; d - share of marginal zone area in the spleen slice; e - number of cells in 1 mm2 of marginal zone; f - number of neutrophils in 1 mm2 of marginal zone. * - from the values of the control group (p < 0.01)

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5. Fig. 4. Morphofunctional characteristics of the spleen red pulp in the first week of life in rats of the control group and rats developed under the influence of low doses of DDT (M ± m): a - total number of cells in 1 mm2 of the red pulp; b - number of neutrophils in 1 mm2 of the red pulp; c - number of megakaryocytes in 1 mm2 of the red pulp. * - from the values of the control group (p < 0.01)

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6. Fig. 5. Age dynamics of the content of (a) B- and (b) T-lymphocytes in the spleen of rats of the control group and rats developing under the influence of low doses of DDT (M ± m). * - from the values of the control group (p < 0.01)

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7. Fig. 6. Age dynamics of subpopulation composition of T-lymphocytes in the spleen of rats of the control group and rats developing under the influence of low doses of DDT (M ± m). * - from the values of the control group (p < 0.01)

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