Effects of Arctigenin in Proliferation, Migration, and Invasion of Nasopharyngeal Carcinoma 5-8F Cells

  • Авторлар: Huang D.1, Lu R.2, Cai M.3, Meng J.3, He S.3, Zhang Q.4, Meng W.3
  • Мекемелер:
    1. Department of Otorhinolaryngology-Head and Neck Surgery,, Nanjing Tongren Hospital Affiliated to Medical College of Southeast University
    2. Ophthalmology Department, Affiliated Eye Hospital of Nanjing Medical Universit
    3. Department of Otorhinolaryngology-Head and Neck Surgery, Nanjing Tongren Hospital Affiliated to Medical College of Southeast University
    4. Department of Otorhinolaryngology-Head and Neck Surgery and Neck Surgery, Nanjing Tongren Hospital Affiliated to Medical College of Southeast University
  • Шығарылым: Том 23, № 10 (2023)
  • Беттер: 1211-1216
  • Бөлім: Oncology
  • URL: https://kld-journal.fedlab.ru/1871-5206/article/view/694291
  • DOI: https://doi.org/10.2174/1871520623666230228155129
  • ID: 694291

Дәйексөз келтіру

Толық мәтін

Аннотация

Background: Nasopharyngeal carcinoma (NPC) is a malignant tumor of the nasopharynx.

Objective: Here, we aimed to understand better the molecular basis for arctigenin (ARG)'s ability to promote NPC 5-8F cell invasion.

Methods: We tested the effects of several doses of ARG on 5-8F cells that had been cultured in vitro. We estimated the metabolic activity of cells by The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium assay. We examined the influence on cell invasion, and migration using Transwell Evaluation. Real-time polymerase chain reaction analysis was used to determine the relative amounts of epidermal growth factor receptor (EGFR), Janus kinase 2 (JAK2) , and transcriptional activator 3 (STAT 3) mRNA expression. Using western blotting, we looked at the level of phosphorylation of specific proteins like EGFR, phosphorylated EGFR, JAK2, and STAT 3.

Results: Our findings revealed that ARG inhibited NPC 5-8F cell development in a dose-and time-dependent manner. The invasiveness and mobility of 5-8F cells were significantly suppressed when ARG was overexpressed in a tumor development model. Expression levels of EGFR, JAK2, and STAT 3 mRNA were considerably low in the experimental group. As a consequence of being treated with ARG, lower levels of EGFR, p-EGFR, p-JAK2, and p-STAT3 expression were observed.

Conclusion: These results suggest that ARG may prevent NPC 5-8F cells from proliferating, migrating, and invading other tissues. There are a few potential molecular pathways, two of which are the inhibition of EGFR phosphorylation and the reduction of levels of phospho-JAK2 and phospho-STAT3.

Авторлар туралы

Dongdong Huang

Department of Otorhinolaryngology-Head and Neck Surgery,, Nanjing Tongren Hospital Affiliated to Medical College of Southeast University

Email: info@benthamscience.net

Rui Lu

Ophthalmology Department, Affiliated Eye Hospital of Nanjing Medical Universit

Email: info@benthamscience.net

Mingjing Cai

Department of Otorhinolaryngology-Head and Neck Surgery, Nanjing Tongren Hospital Affiliated to Medical College of Southeast University

Email: info@benthamscience.net

Jie Meng

Department of Otorhinolaryngology-Head and Neck Surgery, Nanjing Tongren Hospital Affiliated to Medical College of Southeast University

Email: info@benthamscience.net

Shuangba He

Department of Otorhinolaryngology-Head and Neck Surgery, Nanjing Tongren Hospital Affiliated to Medical College of Southeast University

Email: info@benthamscience.net

Qingxiang Zhang

Department of Otorhinolaryngology-Head and Neck Surgery and Neck Surgery, Nanjing Tongren Hospital Affiliated to Medical College of Southeast University

Email: info@benthamscience.net

Wei Meng

Department of Otorhinolaryngology-Head and Neck Surgery, Nanjing Tongren Hospital Affiliated to Medical College of Southeast University

Хат алмасуға жауапты Автор.
Email: info@benthamscience.net

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