Rhizopogon luteolus and Ganoderma adspersum Extracts Inhibit Invasion through the Crosstalk between Anti-oxidant Activity and Apoptosis Induced by pAKT/Rb


Дәйексөз келтіру

Толық мәтін

Аннотация

Objective:Lung cancer is the primary cause of cancer-related deaths globally. Protein kinase B (AKT) protein is associated with many pathways in non-small cell lung cancer (NSCLC), such as proliferation, migration, invasion, and apoptosis. Mushrooms have a long history of being used in traditional medicine to treat various diseases. Scientists have been exploring the potential of mushrooms for their antioxidant and anticancer properties. In our study, the anti-oxidant, invasion, and apoptosis effects of mushroom extracts were investigated in NSCLC.

Materials and Methods:Non-Small Cell Lung Cancer cell lines H1299, PC-3, and PC-14 were used in our study. After obtaining the extracts of Rhizopogon luteolus and Ganoderma adspersum, IC50 value was calculated as 25.04-11.73-16.54 ng/ul for R. luteolus and 2.97-1.53-1.01 μg/ul for G. adspersum, respectively, in H1299, PC3 and PC14 cell lines. Afterward, proliferative and invasion effects, as well as apoptosis and anti-oxidant effects, were investigated using the IC50 dose. Western blotting was performed to investigate the pathways of these effects.

Results:According to the results of our study, Rhizopogon luteolus and Ganoderma adspersum extracts have anti-proliferative and anti-invasive effects on non-small lung cancer cell lines and induced apoptosis, which has been found to increase the anti-oxidant effect. It was found that this effect was due to cross-talk between antioxidant activity and the AKT-Rb pathway.

Conclusion:We anticipate that Rhizopogon luteolus and Ganoderma adspersum extracts will be effective in cancer treatment by suppressing lung cancer cells via p-Akt and Rb.

Авторлар туралы

Aydın Demiray

Department of Medical Genetic, School of Medicine, Pamukkale University

Хат алмасуға жауапты Автор.
Email: info@benthamscience.net

Ege Karagur

Department of Medical Genetic, School of Medicine, Pamukkale University

Email: info@benthamscience.net

Gulsen Tel-Cayan

Department of Chemistry and Chemical Processing Technologies, Muğla Vocational School, Muğla Sıtkı Koçman University

Email: info@benthamscience.net

Onur Tokgun

Department of Medical Genetic, School of Medicine, Pamukkale University

Email: info@benthamscience.net

Hakan Akca

Department of Medical Genetic, School of Medicine, Pamukkale University

Email: info@benthamscience.net

Mehmet Duru

Department of Chemistry, Faculty of Sciences, Muğla Sıtkı Koçman University

Email: info@benthamscience.net

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