Ultrastructural features of Bacillus cerеus strains isolated from ulcerative colitis

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Abstract

Background: Bacillus cereus is a widespread type of bacilli and the causative agent of a number of diseases. The etiopathogenetic role of B. cereus in ulcerative colitis remains unexplored.

Aim: Detection of ultrastructural features of B. cereus strains associated with ulcerative colitis.

Materials and methods: The ultrastructural features of reference strains of B. cereus and strains of B. cereus isolated from ulcerative colitis were studied dynamically using light and electron (scanning and transmission) microscopy.

Results: It has been shown that reference and freshly isolated B. cereus strains from clinical material, when cultured in vitro , demonstrate a similar ability to spore formation, manifested by the formation of spores of a typical structure consisting of a core, cortex, shell, and exosporium. At the same time, B. cereus strains of clinical origin are characterized by the presence of atypical ribbon-like and lamellar inclusions — ultrastructural features that are absent in the reference strains of B. cereus .

Conclusion: The ultrastructural details found may reflect the ecologically determined features of the sporulation of B. cereus strains of clinical origin. The involvement of ribbon-like and lamellar inclusions in the pathogenesis of ulcerative colitis requires further study. Electron microscopic examination may be useful in the framework of the clinical and bacteriological diagnosis of ulcerative colitis.

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About the authors

Vladimir G. Zhukhovitsky

National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya; Russian Medical Academy of Continuous Professional Education

Author for correspondence.
Email: zhukhovitsky@rambler.ru
ORCID iD: 0000-0002-4653-2446
SPIN-code: 7983-1177

MD, Cand. Sci. (Medicine), Professor

Russian Federation, Moscow; Moscow

Tatyana A. Smirnova

National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya

Email: smiryu@mail.ru
ORCID iD: 0000-0001-7121-635X

MD, Dr. Sci. (Medicine)

Russian Federation, Moscow

Marina A. Sukhina

State Scientific Centre of Coloproctology

Email: marinasukhina@rambler.ru
ORCID iD: 0000-0003-4795-0751
SPIN-code: 9577-5290

Cand. Sci. (Biology)

Russian Federation, Moscow

Margarita V. Zubasheva

National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya

Email: mzubzsheva@mail.ru
ORCID iD: 0000-0001-7330-7343
SPIN-code: 3251-0315

Cand. Sci. (Biology)

Russian Federation, Moscow

Natalya V. Shevlyagina

National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya

Email: nataly-123@list.ru
ORCID iD: 0000-0001-9651-1654
SPIN-code: 8629-5414

MD, Cand. Sci. (Medicine)

Russian Federation, Moscow

Svetlana G. Andreevskaya

National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya

Email: hacaranda@yandex.ru
ORCID iD: 0000-0003-4704-4329
SPIN-code: 8162-2103

MD, Cand. Sci. (Medicine)

Russian Federation, Moscow

Anna A. Kuzmina

National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya

Email: nuynik@mail.ru
ORCID iD: 0000-0002-3515-1891
Russian Federation, Moscow

Matvey I. Yasinovskiy

National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya

Email: myasinovski@mail.ru
ORCID iD: 0000-0002-3122-8054
Russian Federation, Moscow

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2. Fig. 1. Material from 12-hour cultures of the reference strain B. cereus ATCC 10876 ( a ) and the freshly isolated strain B. cereus 177 ( b ). Scanning electron microscopy. Magnification: ×8000 ( a ), ×4000 ( b ).

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3. Fig. 2. Material of 12-hour cultures of freshly isolated B. cereus strains 172 ( a ) and 19/16 ( b ). Scanning electron microscopy. Magnification: ×8000 ( a ), ×16000 ( b ).

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4. Fig. 3. 12-hour culture material of the reference strain B. cereus NCTC 8035. Transmission electron microscopy. Magnification: ×10000 ( a ). Light microscopy. The color is Sudan black. Magnification: ×1000 ( b ).

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5. Fig. 4. Lysed vegetative cells (indicated by arrow) in the material of 24-hour cultures of the reference strain B. cereus ATCC 10876 ( a ) and the freshly isolated strain B. cereus 1208 ( b ). Scanning electron microscopy. Magnification: ×8000 ( a ) and ×4000 ( b ).

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6. Fig. 5. The beginning of the sporulation process of 24-hour cultures of the reference strain B. cereus ATCC 10876 ( a ) and the freshly isolated strain B. cereus 1208 ( b ). Transmission electron microscopy. Magnification: ×25000 ( a ) and ×20000 ( b ). The arrows indicate the prosporous protoplast and the emerging exosporium (white and black arrows, respectively).

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7. Fig. 6. Sporulation of 48-hour cultures of the reference strain B. cereus ATCC 10876 ( a ) and the freshly isolated strain B. cereus 1208 ( b ). Transmission electron microscopy. Magnification: ×30000 ( a ) and 15000 ( b ).

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8. Fig. 7. Sporulation of 72-hour cultures of the reference strain B. cereus ATCC 10876 ( a ) and the freshly isolated strain B. cereus 1208 ( b ). Transmission electron microscopy. Magnification: ×20000 ( a ) and ×25000 ( b ).

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9. Fig. 8. Spores of 96-hour cultures of the reference strain B. cereus ATCC 10876 ( a ) and the freshly isolated strain B. cereus 181 ( b ). Scanning electron microscopy. Magnification: ×16000 ( a ) and ×30000 ( b ). The arrows indicate the exosporium.

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10. Fig. 9. Ultrastructure of the spore of a 96-hour culture of a freshly isolated strain of B. cereus 181. Transmission electron microscopy. Magnification: ×80000; structural units ( a ) and absolute dimensions in nm ( b ).

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11. Fig. 10. Atypical cell structures of a 72-hour culture of a freshly isolated strain of B. cereus 172. Transmission electron microscopy. Magnification: ×50000. The arrows indicate ribbon-like structures in the spore and in the cytoplasm of the sporangium (white and black arrows, respectively).

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12. Fig. 11. Atypical cell structures of a 72-hour culture of a freshly isolated strain of B. cereus 214. Transmission electron microscopy. Magnification: ×40000 ( a ) and ×60000 ( b ). The arrows indicate lamellar structures in the exosporeal space and outside the spore (white and black arrows, respectively).

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Copyright (c) 2024 Zhukhovitsky V.G., Smirnova T.A., Sukhina M.A., Zubasheva M.V., Shevlyagina N.V., Andreevskaya S.G., Kuzmina A.A., Yasinovskiy M.I.

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