Ultrastructural features of Bacillus cerеus strains isolated from ulcerative colitis

Мұқаба


Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Рұқсат ақылы немесе тек жазылушылар үшін

Аннотация

Background: Bacillus cereus is a widespread type of bacilli and the causative agent of a number of diseases. The etiopathogenetic role of B. cereus in ulcerative colitis remains unexplored.

Aim: Detection of ultrastructural features of B. cereus strains associated with ulcerative colitis.

Materials and methods: The ultrastructural features of reference strains of B. cereus and strains of B. cereus isolated from ulcerative colitis were studied dynamically using light and electron (scanning and transmission) microscopy.

Results: It has been shown that reference and freshly isolated B. cereus strains from clinical material, when cultured in vitro , demonstrate a similar ability to spore formation, manifested by the formation of spores of a typical structure consisting of a core, cortex, shell, and exosporium. At the same time, B. cereus strains of clinical origin are characterized by the presence of atypical ribbon-like and lamellar inclusions — ultrastructural features that are absent in the reference strains of B. cereus .

Conclusion: The ultrastructural details found may reflect the ecologically determined features of the sporulation of B. cereus strains of clinical origin. The involvement of ribbon-like and lamellar inclusions in the pathogenesis of ulcerative colitis requires further study. Electron microscopic examination may be useful in the framework of the clinical and bacteriological diagnosis of ulcerative colitis.

Толық мәтін

Рұқсат жабық

Авторлар туралы

Vladimir Zhukhovitsky

National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya; Russian Medical Academy of Continuous Professional Education

Хат алмасуға жауапты Автор.
Email: zhukhovitsky@rambler.ru
ORCID iD: 0000-0002-4653-2446
SPIN-код: 7983-1177

MD, Cand. Sci. (Medicine), Professor

Ресей, Moscow; Moscow

Tatyana Smirnova

National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya

Email: smiryu@mail.ru
ORCID iD: 0000-0001-7121-635X

MD, Dr. Sci. (Medicine)

Ресей, Moscow

Marina Sukhina

State Scientific Centre of Coloproctology

Email: marinasukhina@rambler.ru
ORCID iD: 0000-0003-4795-0751
SPIN-код: 9577-5290

Cand. Sci. (Biology)

Ресей, Moscow

Margarita Zubasheva

National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya

Email: mzubzsheva@mail.ru
ORCID iD: 0000-0001-7330-7343
SPIN-код: 3251-0315

Cand. Sci. (Biology)

Ресей, Moscow

Natalya Shevlyagina

National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya

Email: nataly-123@list.ru
ORCID iD: 0000-0001-9651-1654
SPIN-код: 8629-5414

MD, Cand. Sci. (Medicine)

Ресей, Moscow

Svetlana Andreevskaya

National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya

Email: hacaranda@yandex.ru
ORCID iD: 0000-0003-4704-4329
SPIN-код: 8162-2103

MD, Cand. Sci. (Medicine)

Ресей, Moscow

Anna Kuzmina

National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya

Email: nuynik@mail.ru
ORCID iD: 0000-0002-3515-1891
Ресей, Moscow

Matvey Yasinovskiy

National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya

Email: myasinovski@mail.ru
ORCID iD: 0000-0002-3122-8054
Ресей, Moscow

Әдебиет тізімі

  1. Liu Y, Lai Q, Göker M, et al. Genomic insights into the taxonomic status of the Bacillus cereus group. Scientific Reports. 2015(5):14082. doi: 10.1038/srep14082
  2. Bazinet AL. Pan-genome and phylogeny of Bacillus cereus sensu lato. BMC Ecology and Evolution. 2017;17:176–191. doi: 10.1186/s12862-017-1020-1
  3. Jensen GB, Hansen BM, Eilenberg J, Mahillon J. The hidden lifestyles of Bacillus cereus and relatives. Environ Microbiol. 2003;5(8):631–640. doi: 10.1046/j.1462-2920.2003.00461.x
  4. Logan NA, Hoffmaster AR, Shadomy SV, Stauffer KE. Bacillus and other aerobic endospore-forming bacteria. Versalovic J, Karroll KC, Funke G, et al editors. Washington: ASM Press; 2011.
  5. Rasko DA, Altherr MR, Han CS, Ravel J. Genomics of the Bacillus cereus group of organisms. FEMS Microbiol Rev. 2005;29(2)303–329. doi: 10.1016/j.femsre.2004.12.005
  6. Kolstø AB, Lereclus D, Mock M. Genome structure and evolution of the Bacillus cereus group. Curr. Top. Microbiol. Immunol. 2002;264(2):95–108.
  7. Vasilev DA, Kaldyrkaev AI, Feoktistova NA, Aleshkin AV. Identification of bacillus cereus bacteria based on their phenotypic characteristic. Ulyanovsk: Color-Print LLC; 2013. EDN: WYWEXJ
  8. Guinebretière M-H, Thompson FL, Sorokin A, et al. Ecological diversification in the Bacillus cereus Group. Environ. Microbiol. 2008;10(4):851–865. doi: 10.1111/j.1462-2920.2007.01495.x
  9. Quagliariello A, Cirigliano A, Rinaldi T. Bacilli in the International Space Station. Microorganisms. 2022;10(12):2309–2323. doi: 10.3390/microorganisms10122309
  10. Heini N, Stephan R, Ehling-Schulz M, Johler S. Characterization of Bacillus cereus group isolates from powdered food products. International Journal of Food Microbiology. 2018(283):59–64. doi: 10.1016/j.ijfoodmicro.2018.06.019
  11. Okinaka RT, Keim P. The Phylogeny of Bacillus cereus sensu lato. Microbiol Spectr. 2016;4(1). doi: 10.1128/microbiolspec.TBS-0012-2012
  12. Hakovirta JR, Prezioso S, Hodge D, et al. Identification and Analysis of Informative Single Nucleotide Polymorphisms in 16S rRNA Gene Sequences of the Bacillus cereus Group. Journal of Clinical Microbiology. 2016;54(11):2749–2756. doi: 10.1128/JCM.01267-16
  13. Bianco A, Capozzi L, Miccolupo A, et al. Multi-locus sequence typing and virulence profile in Bacillus cereus sensu lato strains isolated from dairy products. Italian Journal of Food Safety. 2020;9:8401. doi: 10.4081/ijfs.2020.8401
  14. Manzulli V, Rondinone V, Buchicchio A, et al. Discrimination of Bacillus cereus Group Members by MALDI-TOF Mass Spectrometry. Microorganisms. 2021;9(6):1202. doi: 10.3390/microorganisms9061202
  15. Laue M, Fulda G. Rapid and reliable detection of bacterial endospores in environmental samples by diagnostic electron microscopy combined with X-ray microanalysis. Journal of Microbiological Methods. 2013;94(1):13–21. doi: 10.1016/j.mimet.2013.03.026
  16. Ceuppens S, Boon N, Uyttendaele M. Diversity of Bacillus cereus group strains is reflected in their broad range of pathogenicity and diverse ecological lifestyles. FEMS Microbiology Ecology. 2013;84(3):433–450. doi: 10.1111/1574-6941.12110
  17. Ehling-Schulz M, Lereclus D, Koehler TM. The Bacillus cereus Group: Bacillus Species with Pathogenic Potential. Microbiology Spectrum. 2019;7(3):GPP3-0032-2018. doi: 10.1128/microbiolspec.GPP3-0032-2018
  18. Sastalla I, Fattah R, Coppage N, et al. The Bacillus cereus Hbl and Nhe Tripartite Enterotoxin Components Assemble Sequentially on the Surface of Target Cells and Are Not Interchangeable. PLoS One. 2013;8(10):e76955. doi: 10.1371/journal.pone.0076955
  19. Ehling-Schulz M, Frenzel E, Gohar M. Food–bacteria interplay: pathometabolism of emetic Bacillus cereus . Front. Microbiol. 2015;14(6):704. doi: 10.3389/fmicb.2015.00704
  20. Tuipulotu DE, Mathur A, Ngo C, Man SM. Bacillus cereus : Epidemiology, Virulence Factors, and Host–Pathogen Interactions. Trends Microbiol. 2021;29(5):458–471. doi: 10.1016/j.tim.2020.09.003
  21. Ikram S, Heikal A, Finke S, et al. Bacillus cereus biofilm formation on central venous catheters of hospitalised cardiac patients. Biofouling. 2019;35(2):204–216. doi: 10.1080/08927014.2019.1586889
  22. Bottone EJ. Bacillus cereus , a Volatile Human Pathogen. Clinical Microbiology Reviews. 2010;23(2):382–398. doi: 10.1128/CMR.00073-09
  23. Wiedbrauk DL. Microscopy. Versalovic J, Karroll KC, Funke G, et al editors. Washington: ASM Press; 2011.
  24. Deutsch R. Identification methods in light microscopy chapter 3. Gerhard F, editor. Moscow: The world; 1983.
  25. Ito S, Karnovsky M. Formaldehyde-Glutaraldehyde Fixatives Containing Trinitrocompounds. The Journal of Cell Biology. 1968;39:168A–169A.
  26. Bressuire-Isoard C, Broussolle V, Carlin F. Sporulation environment influences spore properties in Bacillus : evidence and insights on underlying molecular and physiological mechanisms. FEMS Microbiology Reviews. 2018;42(5):614–626. doi: 10.1093/femsre/fuy021
  27. Plieva ZS, Smirnova TA, Zubasheva МV, et al. Structure of exosporium of spores of Bacillus cereus . Nanotechnologies. 2020;13(7–8):426–432. doi: 10.22184/1993-8578.2020.13.7-8.426.432

Қосымша файлдар

Қосымша файлдар
Әрекет
1. JATS XML
Жүктеу
2. Fig. 1. Material from 12-hour cultures of the reference strain B. cereus ATCC 10876 ( a ) and the freshly isolated strain B. cereus 177 ( b ). Scanning electron microscopy. Magnification: ×8000 ( a ), ×4000 ( b ).

Жүктеу (402KB)
Метадеректер
3. Fig. 2. Material of 12-hour cultures of freshly isolated B. cereus strains 172 ( a ) and 19/16 ( b ). Scanning electron microscopy. Magnification: ×8000 ( a ), ×16000 ( b ).

Жүктеу (347KB)
Метадеректер
4. Fig. 3. 12-hour culture material of the reference strain B. cereus NCTC 8035. Transmission electron microscopy. Magnification: ×10000 ( a ). Light microscopy. The color is Sudan black. Magnification: ×1000 ( b ).

Жүктеу (494KB)
Метадеректер
5. Fig. 4. Lysed vegetative cells (indicated by arrow) in the material of 24-hour cultures of the reference strain B. cereus ATCC 10876 ( a ) and the freshly isolated strain B. cereus 1208 ( b ). Scanning electron microscopy. Magnification: ×8000 ( a ) and ×4000 ( b ).

Жүктеу (266KB)
Метадеректер
6. Fig. 5. The beginning of the sporulation process of 24-hour cultures of the reference strain B. cereus ATCC 10876 ( a ) and the freshly isolated strain B. cereus 1208 ( b ). Transmission electron microscopy. Magnification: ×25000 ( a ) and ×20000 ( b ). The arrows indicate the prosporous protoplast and the emerging exosporium (white and black arrows, respectively).

Жүктеу (322KB)
Метадеректер
7. Fig. 6. Sporulation of 48-hour cultures of the reference strain B. cereus ATCC 10876 ( a ) and the freshly isolated strain B. cereus 1208 ( b ). Transmission electron microscopy. Magnification: ×30000 ( a ) and 15000 ( b ).

Жүктеу (779KB)
Метадеректер
8. Fig. 7. Sporulation of 72-hour cultures of the reference strain B. cereus ATCC 10876 ( a ) and the freshly isolated strain B. cereus 1208 ( b ). Transmission electron microscopy. Magnification: ×20000 ( a ) and ×25000 ( b ).

Жүктеу (300KB)
Метадеректер
9. Fig. 8. Spores of 96-hour cultures of the reference strain B. cereus ATCC 10876 ( a ) and the freshly isolated strain B. cereus 181 ( b ). Scanning electron microscopy. Magnification: ×16000 ( a ) and ×30000 ( b ). The arrows indicate the exosporium.

Жүктеу (205KB)
Метадеректер
10. Fig. 9. Ultrastructure of the spore of a 96-hour culture of a freshly isolated strain of B. cereus 181. Transmission electron microscopy. Magnification: ×80000; structural units ( a ) and absolute dimensions in nm ( b ).

Жүктеу (418KB)
Метадеректер
11. Fig. 10. Atypical cell structures of a 72-hour culture of a freshly isolated strain of B. cereus 172. Transmission electron microscopy. Magnification: ×50000. The arrows indicate ribbon-like structures in the spore and in the cytoplasm of the sporangium (white and black arrows, respectively).

Жүктеу (221KB)
Метадеректер
12. Fig. 11. Atypical cell structures of a 72-hour culture of a freshly isolated strain of B. cereus 214. Transmission electron microscopy. Magnification: ×40000 ( a ) and ×60000 ( b ). The arrows indicate lamellar structures in the exosporeal space and outside the spore (white and black arrows, respectively).

Жүктеу (447KB)
Метадеректер

© Zhukhovitsky V.G., Smirnova T.A., Sukhina M.A., Zubasheva M.V., Shevlyagina N.V., Andreevskaya S.G., Kuzmina A.A., Yasinovskiy M.I., 2024

Creative Commons License
Бұл мақала лицензия бойынша қол жетімді Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ:  ПИ № ФС 77 - 86785 от 05.02.2024 (ранее — ПИ № ФС 77 - 59057 от 22.08.2014).