Antibiotic resistance of obligate anaerobes isolated from patients with coloproctological diseases

Alina R. Melkumyan; Мелкумян Алина Рантиковна; Sergey I. Achkasov; Ачкасов Сергей Иванович; Anastasia A. Shafikova; Шафикова Анастасия Александровна; Daria A. Chistyakova; Чистякова Дарья Алексеевна; Mikhail V. Spivak; Спивак Михаил Владимирович; Olga E. Orlova; Орлова Ольга Евгеньевна; Svetlana A. Gordeeva; Гордеева Светлана Александровна; Zhanna V. Khamtsova; Хамцова Жанна Валерьевна; Antonina V. Vetokhina; Ветохина Антонина Владиславовна

doi:10.17816/cld640813

Antibiotic resistance of obligate anaerobes isolated from patients with coloproctological diseases

作者: Melkumyan A.R.¹^,2, Achkasov S.I.¹^,2, Shafikova A.A.¹, Chistyakova D.A.¹, Spivak M.V.¹, Orlova O.E.³, Gordeeva S.A.⁴, Khamtsova Z.V.⁵, Vetokhina A.V.⁶
隶属关系:
1. State Scientific Centre of Coloproctology named after A.N. Ryzhih
2. Russian Medical Academy of Continuous Professional Education
3. City Clinical Hospital No. 67 named after L.A. Vorohobov
4. Clinical Infectious Diseases Hospital named after S.P. Botkin
5. Center for Specialized Types of Medical Care named after. V.P. Avaev
6. Irkutsk Order of the Badge of Honor Regional Clinical Hospital
期: 卷 69, 编号 9 (2024)
页面: 175-185
栏目: Original Study Articles
##submission.datePublished##: 18.02.2024
URL: https://kld-journal.fedlab.ru/0869-2084/article/view/640813
DOI: https://doi.org/10.17816/cld640813
ID: 640813

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BACKGROUND: Anaerobic infections are life-threatening, often severe, and dangerous for patients with comorbidities. However, in the routine practice of microbiological laboratories, anaerobes are usually not cultured or identified, and the susceptibility of obligate anaerobes to antibiotics is rarely determined. However, antibiotic resistance has increased worldwide, including among anaerobic bacteria.

AIM: The aim of the study was to evaluate the susceptibility of etiologically significant species of obligate anaerobic bacteria isolated from patients with coloproctological diseases from different regions of the Russian Federation.

MATERIALS AND METHODS: The multicenter, prospective, uncontrolled study included etiologically significant obligate anaerobes isolated from patients with coloproctological diseases in 65 regions of the Russian Federation. Subsequent evaluation of antibiotic susceptibility was performed in accordance with the regulatory documents used in the Russian Federation.

RESULTS: As a result of the study, 1,200 strains of obligate anaerobes of 123 species were collected. The analysis included the results of susceptibility testing of 430 anaerobic strains causing proctological infections, including Bacteroides (n =202), Parabacteroides (n =22), Phocaeicola vulgatus (n =104), Prevotella (n =11), Clostridium perfringens (n =91). Resistance to metronidazole, clindamycin, meropenem and vancomycin was detected in 77.3%, 72.7%, 62.5% and 8.79% of the strains, respectively.

CONCLUSION: Monitoring for antibiotic resistance in obligate anaerobes that pose a threat of spreading antibiotic-resistant strains helps reduce the spread of resistance and personalizes antimicrobial therapy and prevention protocols for patients, reducing treatment costs and hospital stay time by quickly switching from empiric to etiotropic therapy.

关键词

anaerobic bacteria, antibacterial agents, drug resistance of microorganisms, bacterial drug resistance, surgical wound infection, postoperative wound infection, postoperative complications

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作者简介

Alina Melkumyan

State Scientific Centre of Coloproctology named after A.N. Ryzhih; Russian Medical Academy of Continuous Professional Education

编辑信件的主要联系方式.
Email: alinamelkumyan@yandex.ru
ORCID iD: 0000-0002-5494-415X
SPIN 代码: 2313-8930

MD, Cand. Sci. (Medicine)

俄罗斯联邦, 2 Salyama Adilya street, 123423 Moscow; Moscow

Sergey Achkasov

State Scientific Centre of Coloproctology named after A.N. Ryzhih; Russian Medical Academy of Continuous Professional Education

Email: info@gnck.ru
ORCID iD: 0000-0001-9294-5447
SPIN 代码: 5467-1062

MD, Dr. Sci. (Medicine), Professor, corresponding member of the Russian Academy of Sciences

俄罗斯联邦, 2 Salyama Adilya street, 123423 Moscow; Moscow

Cand. Sci. (Biology)

俄罗斯联邦, Moscow

参考

Brown SR, Fearnhead NS, Faiz OD, et al. The Association of Coloproctology of Great Britain and Ireland consensus guidelines in surgery for inflammatory bowel disease. Colorectal Dis. 2018;20 Suppl 8:3–117. doi: 10.1111/codi.14448
Brusina EB, Rychagov IP. Epidemiology of nosocomial purulent-septic infections in surgery. Moscow : Nauka; 2006. 168 p. EDN: SJHPUP
Feldblyum IV, Zakharova YuA. Nosocomial infections: issues of terminology and modern classification. Epidemiology and Vaccinal Prevention. 2009;1:19–24. EDN: KHOITV
Zueva LP, Yafaev RKh. Epidemiology: textbook. St. Petersburg: Foliot, 2005. 752 p.
Malashenko AA, Aslanov BI, Nechaev VV. Active Epidemiological Surveillance: the Key for Effective Infection Prevention in Pediatric Surgery. Epidemiology and Vaccinal Prevention. 2018;17(6):76–80. doi: 10.31631/2073-3046-2018-17-76-80
Martin D, Hübner M, Moulin E, et al. Timing, diagnosis, and treatment of surgical site infections after colonic surgery: prospective surveillance of 1263 patients. J Hosp Infect. 2018;100(4):393–399. doi: 10.1016/j.jhin.2018.09.011
K B, R S, J S, et al. Surgical Site Infections in Gastrointestinal Surgeries: Estimation of Prevalence, Risk Factors and Bacteriological Profile. Cureus. 2024;16(6):e62589. doi: 10.7759/cureus.62589
Nagy E, Boyanova L, Justesen US, ESCMID Study Group of Anaerobic Infections. How to isolate, identify and determine antimicrobial susceptibility of anaerobic bacteria in routine laboratories. Clin Microbiol Infect. 2018;24(11):1139–1148. doi: 10.1016/j.cmi.2018.02.008
Schuetz AN. Antimicrobial resistance and susceptibility testing of anaerobic bacteria. Clin Infect Dis. 2014;59(5):698–705. doi: 10.1093/cid/ciu395
Nagy E, Urbán E, Nord CE, ESCMID Study Group on Antimicrobial Resistance in Anaerobic Bacteria. Antimicrobial susceptibility of Bacteroides fragilis group isolates in Europe: 20 years of experience. Clin Microbiol Infect. 2011;17(3):371–379. doi: 10.1111/j.1469-0691.2010.03256.x
Wright GD. The antibiotic resistome: the nexus of chemical and genetic diversity. Nat Rev Microbiol. 2007;5(3):175–186. doi: 10.1038/nrmicro1614
Salyers AA, Gupta A, Wang Y. Human intestinal bacteria as reservoirs for antibiotic resistance genes. Trends Microbiol. 2004;12(9):412–416. doi: 10.1016/j.tim.2004.07.004
Cooley L, Teng J. Anaerobic resistance: should we be worried? Curr Opin Infect Dis. 2019;32(6):523–530. doi: 10.1097/QCO.0000000000000595
Russian recommendations. Determination of the sensitivity of microorganisms to antimicrobial drugs. Version 2024-02. MAKMAKH, SSMU: Smolensk; 2024. 192 p. (In Russ.)
Kuzmenkov AY, Trushin IV, Vinogradova AG, et al. AMRmap: An Interactive Web Platform for Analysis of Antimicrobial Resistance Surveillance Data in Russia. Front Microbiol. 2021;12:620002. doi: 10.3389/fmicb.2021.620002
Vinogradova AG, Kuzmenkov AYu. Application of AMRmap: «from the general to the specific» approach by the example of Klebsiella pneumoniae. Clinical Microbiology and Antimicrobial Chemotherapy. 2019;21(2):181–186. doi: 10.36488/cmac.2019.2.181-186

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2. Fig. 1. Distribution of patients by place of residence.

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3. Fig. 2. The number of patients, in accordance with the diagnoses according to ICD-10, from whom strains of the collection of obligate anaerobic microorganisms were obtained.